Training
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BOD seed
Seed preparation and addition De-ionized water kills - Adding a seed capsule to deionized water causes an osmotic imbalance that will kill seed organisms. The purified water —containing no salts— rushes across the bacterial cell walls. The bacteria…
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Sampling and sample handling for BOD
Water and wastewater samples are biologically active, and the BOD will quickly deteriorate. Minimize the time between sampling and analysis to obtain the best BOD results. Take the following precautions when sampling and handling BOD samples.…
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BOD resources
Websites Field Training Manual for Laboratory Analysts: Chapter 7 - BOD Biochemical Oxygen Demand Source: http://home.windstream.net/mikeric/Chap1to9/Chap7%20Biochemical%20Oxygen%20Demand.htm-->
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BOD quality control
Limit of detection (LOD) BOD detection limits are theoretically based. A key assumption which the concept of detection for BOD is based is that the LEAST amount of depletion allowable is 2 mg/L. The LOD is also based on the highest volume of sample…
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BOD sample pre-treatment
Because the BOD test is a bioassay, it is critical to maintain optimal conditions for the "bugs" (bacteria and other microorganisms) to remain viable. Temperature, pH, oxygen levels and the presence of disinfectants can all influence the outcome of…
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Method Details
The following are the critical steps in preparing and analyzing samples for BOD testing. The BOD test is a method-defined (empirical) technique. Consequently, the method must be followed carefully, and must be performed consistently from day to day…
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BOD dilution water
BOD dilution water nutrient solutions can be a source of contamination. If you prepare your own solution, make sure you store the phosphate buffer in a refrigerator. Discard any solution if it becomes cloudy or you observe any "chunks" floating in…
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Calibration & barometric pressure
Pressure adjustments Typically, any barometric pressure measurement that you hear (radio, TV) represents a value that has been corrected to a value relative to sea level. Why is this? For one thing, it's a means of standardizing data. Barometric…
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DO meter calibration
Calibration of the DO meter is arguably the most critical aspect of BOD testing, since we measure the consumption of dissolved oxygen (DO). While calibration may be the center of the DO measurement universe, calibration is in turn impacted by both…